Job Description

The position is for highly qualified Rsch Pro 6 Biomedical Engineering with at least 6 years of post-PhD lab experience in biochemistry, molecular biology, biophysics, quantitative biology, synthetic biology.

RESEARCH DESCRIPTION:
The Rsch Pro 6-Biomedical Engineering will perform quantitative experiments related to cell-free transcription-translation, cell-free phage synthesis and phage genome engineering, biophysics measurements using fluorescence microscopy and a quartz crystal microbalance with dissipation (QCMD) with cell-free reactions. The Rsch Pro 6-Biomedical Engineering must know how to prepare and quantitively use cell-free transcription-translation systems from E. coli, use a QCMD, perform quantitative measurements using fluorescence microscopy, SDS PAGES, DNA electrophoresis, DNA technologies (DNA assembly, sequencing).

JOB DESCRIPTION:
80% Research
This position will develop research plans, and solve highly complex problems through the original design of protocols, policies, and procedures. The research subject is proposed by the incumbent and decided in agreement with supervisor to be inline with general orientation with other research activities within the Noireaux biomedical physics research group.
Prepare and quantitively use cell-free transcription-translation systems from E. coli
Use a quartz crystal microbalance with dissipation
Perform quantitative measurements using fluorescence microscopy, SDS PAGES, DNA electrophoresis, DNA technologies (DNA assembly, sequencing).
Analyze, interpret, plot the data for all the experiments

10% Communicating Research Results
Maintaining familiarity with cutting-edge results and methods.
Attending meetings, seminars and conferences
Attending weekly and monthly lab meetings
Train younger lab members when needed
Communicating research to the broader community
Writing papers and submitting them for publication in scientific journals
Presenting results at conferences (both domestic and international)

10% Lab maintenance 
Ordering research supplies
Lab cleaning
Equipment and Instrument maintenance

Other duties of a similar scope as assigned.

REQUIRED QUALIFICATIONS:
Ph.D. in biophysics, biochemistry, synthetic biology or related field.
The Rsch Pro 6: 
- must have shown expertise to prepare cell-free transcription-translation systems from diverse E. coli strains with capabilities of at least 3 mg/ml of protein synthesis.
- must have shown capabilities to utilize acoustic liquid dispensers, plate readers, spectrometers, to perform cell-free gene expression reactions and quantitative fluorescent reporter protein measurements.
- must have shown abilities to make SDS PAGES for protein analysis, DNA assembly for cloning (Gibson, MoClo) and sequencing.
- must know how to use a cell-free gene expression on quartz crystal microbalance with dissipation for membrane biophysics measurements.
- must know all the fundamental phage assays: plaque assay, spotting assay, phage infection assay.
- must know how to use fluorescence microscope and make quantitative fluorescence measurements of cell-free gene expression reaction samples.

PREFERRED QUALIFICATIONS: 
In addition to the listed required qualifications, the following qualifications are highly recommended:
- soft matter physics using lipids (liposome formation)
- TLC chromatography

About University of Minnesota Twin Cities

The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.

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